Table 4.

Biotin degrees of labeling was estimated with different incubation time (challenge ratio = 12:1 in the dark).

Biotin DOL incubation time	1 h	2 h	4 h
0Biotin-protein (Not Labeled)	0.05	0.03	ND
1Biotin-protein	0.24	0.03	0.04
2Biotin-protein	0.42	0.20	0.07
3Biotin-protein	0.20	0.22	0.08
4Biotin-protein	0.07	0.34	0.15
5Biotin-protein	0.02	0.09	0.38
6Biotin-protein	ND	0.06	0.17
7Biotin-protein	ND	0.04	0.11
Averaged Biotin DOL	2.0	3.5	4.7

Most abundant biotin incorporation frequency highlighted in bold.

DOL: Degrees of labeling.