Extended Data Fig. 5. F_st around ACD6 and globally.

a, Cockerham and Weir’s fixation index F_st was estimated for SNPs in a list of known immune-associated genes. The genome-wide most extreme F_st values are concentrated in a region on chromosome 4 that includes the immune regulator ACD6 (At4g14400). Reference genome positions (in nt) on chromosome 4 given at the bottom. b, Bergelson and colleagues³² identified A. thaliana SNPs associated with (and likely to influence) microbiome composition. We compared the geographic differentiation of these SNPs (F_st) to the genome-wide distribution. Microbiome-associated SNPs exhibit significantly higher F_st values than the remainder of the genomic SNPs (Wilcoxon rank-sum test p < 2.2x10⁻¹⁶). The central horizontal line in each box indicates the median, the bounds indicate the upper and lower quartiles and the whiskers indicate 1.5*inner quartile range.