Key resource table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse Anti-HA Tag Antibody | Biolegend | Cat# 901516 |
| Rabbit Anti-Bip | BEI Resources | Cat# MRA-1246 |
| HRP Secondary Antibody Rabbit | Abcam | Cat# ab205718 |
| HRP Secondary Antibody Mouse | Abcam | Cat# ab205719 |
| Bacterial and virus strains | ||
| E. coli BL21DE3 | New England Biolabs | Cat# C2527 |
| Chemicals, peptides, and recombinant proteins | ||
| Mixed Alkyl/Aryl Phosphonate Library | This Paper | N/A |
| Recombinant human monoacylglycerol lipase (MAGL) | Cayman Chemical | Cat# 10007812 |
| Recombinant P. falciparum PfMAGLLP | Yoo et al.12 | N/A |
| 4-Methylumbelliferyl caprylate | Santa Cruz Biotech. | Cat# sc-281420 |
| Fluorophosphonate-rhodamine (FP-Rho) | Lentz et al.62 | N/A |
| Orlistat | Cayman Chemical | Cat#10005426 |
| YOYO-1 iodide | Thermo Fisher | Cat# Y3601 |
| anhydrotetracycline | Cayman Chemical | Cat#10009542 |
| Critical commercial assays | ||
| Cell Titer Blue Cell Viability Assay Kit | Promega | Cat# G8080 |
| BCA protein assay kit | Pierce | Cat# 23225 |
| Bradford assay kit | ThermoFisher | Cat# 23200 |
| Deposited data | ||
| Mass spectrometry proteomics (PRIDE) | This Paper | https://www.ebi.ac.uk/pride/ ; Accession number: PXD051499 |
| Mass spectrometry lipidomics (DRYAD) | This Paper |
https://datadryad.org/stash doi:10.5061/dryad.b5mkkwhmj |
| Experimental models: Cell lines | ||
| Human foreskin fibroblast (HFF) | John Boothroyd Lab | N/A |
| Experimental models: Organisms/strains | ||
| P. falciparum strain W2 | MR4 | Cat # MRA-157 |
| P. falciparum strain 3D7 | MR4 | Cat # MRA-102 |
| P. falciparum abH112-cMUT | Davison et al.22 | N/A |
| P. falciparum strain NF54 | MR4 | Cat # MRA-1000 |
| P. falciparum strain NF54 abH112 cKD | This Paper | N/A |
| P. falciparum strain Dd2 PfPRELI T145I | Yoo et al.12 | N/A |
| P. falciparum strain Dd2 PfPRELI P102L | Yoo et al.12 | N/A |
| P. falciparum strain Dd2_Polδ | Yoo et al.12 | N/A |
| Oligonucleotides | ||
| AGAGGTTCGACGGAAGTATCTGTTATAATG (P1) | Eurofins | N/A |
| ACAACTCCAGTGAAAAGTTCTTCTCCT (P2) | Eurofins | N/A |
| ATCATTCTTTCCATGCATAATAAATAAAGG (P3) | Eurofins | N/A |
| GTGAGTACATAAATATATTATATAAACTAGACTAGGTTAACTGGCCAAGATCTCCCGGG (P8408) | Eurofins | N/A |
| TCCTCCTCAGAAATTAGCTTCTGCTC (P8470) | Eurofins | N/A |
| GTTGAGTTGGGAAAATACTGGTGAACTAG (P8510) | Eurofins | N/A |
| CGGGTTTAGGGGACGCATGG (P8977) | Eurofins | N/A |
| GCTTTTATCACTCAAGCGAAAAGCATGAA (P8978) | Eurofins | N/A |
| AGTTAAAATAAGGCTAGTCCGTTATCAACTTG (P9141) | Eurofins | N/A |
| taatacgactcactataggGAGCTATTTTATCATAAATTgttttagagctagaaatag (Guide) | Eurofins | N/A |
| Software and algorithms | ||
| MaxQuant | Max Planck Institute of Biochemistry | https://maxquant.net/perseus/ |
| Perseus | European Molecular Biology Laboratory | https://www.ebi.ac.uk/jdispatcher/msa/clustalo |
| Clustal Omega | Seivers et al.42 | https://www.ebi.ac.uk/jdispatcher/msa/clustalo |
| Alphafold | Jumper et al.46 | https://alphafold.ebi.ac.uk/ |
| PyMol | The PyMOL Molecular Graphics System, Schrodinger, LLC | https://pymol.org/ |
| GraphPad Prism | GraphPad Software Inc | https://www.graphpad.com/scientific-software/prism/ |