FIG. 1.

Characterization of the SrCap-specific monoclonal antibody that was raised against the 239-aa carboxyl terminus of the SrCap protein. Either purified His-tagged SrCap carboxyl terminus (lane 1), total cell protein from bacterial lysates (lane 2), or bacterial lysates containing a GST-tagged SrCap fusion protein (lane 3) were resolved by SDS–12% PAGE and transferred to a Western blot membrane. Both His- and GST-tagged SrCap proteins contained the carboxyl-terminal 239 aa of SrCap. This membrane was probed with the SrCap-specific antibody. Positions of His-GST fusion proteins and molecular mass markers are indicated on the left and right, respectively.