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. 2001 Nov;75(21):10033–10040. doi: 10.1128/JVI.75.21.10033-10040.2001

FIG. 5.

FIG. 5

The 72-kDa protein present in the SrCap-specific coimmunoprecipitation is identified as the adenovirus DNA binding protein, DBP. (A) Proteins from uninfected (−) or adenovirus-infected (+) A549 cell lysates were immunoprecipitated with the SrCap-specific or DBP-specific monoclonal antibodies and resolved by SDS-PAGE and then transferred to a PVDF Western blot membrane. The membrane was probed with a DBP-specific monoclonal antibody. The position of DBP is indicated to the left. Control antibody was specific for the retinoblastoma gene product. (B) Proteins from uninfected (−) or adenovirus-infected (+) A549 cell lysates were immunoprecipitated with the SrCap-specific or DBP-specific monoclonal antibodies. These immune complexes were denatured (as described in Materials and Methods) and reimmunoprecipitated with a DBP-specific monoclonal antibody. The position of DBP is indicated to the left.