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. 2024 Aug 6;9(18):e179292. doi: 10.1172/jci.insight.179292

Figure 2. Deep profiling of myeloid, dendritic, and lymphoid cells by CyTOF in subcutaneous KPCY 6419c5 model.

Figure 2

(A) Uniform manifold approximation and projection (UMAP) and heatmap of final annotated clusters based on relative staining intensities of canonical subtyping markers. UMAP shows 2,000 cells per sample. B, B cell; Th, helper T cell; Tc, cytotoxic T cell; DC, dendritic cell; NK, natural killer cell; TAM, tumor-associated macrophage; Gran, granulocyte; MMDSC, monocytic myeloid-derived suppressor cell; UA, unassigned. (B–D) Boxplots showing mean metal intensity (MMI) of selected functional markers within selected clusters (n = 14). C, control; I, ICIs (anti–PD-1 & anti–CTLA-4); IT, ICIs + tadalafil; L, listeria vaccine; LI, listeria vaccine + ICIs; LIT, listeria vaccine + ICIs + tadalafil. For FDR-adjusted P values: *P ≤ 0.05, for unadjusted P values: #P ≤ 0.05, by 2-tailed Student’s t test between groups of interest.