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. 2024 Aug 13;9(18):e180114. doi: 10.1172/jci.insight.180114

Figure 6. Metabolic cellular crosstalk between epithelial cells and TAMs.

Figure 6

(A) Violin plots showing ABCG1 is significantly upregulated in TAMs, and LDLR is significantly upregulated in tumor-derived epithelial cells. (B) Schematic of TAMs increasing ABCG1 (cholesterol exporter) expression. Cancer cells increase expression of a corresponding lipid/cholesterol receptor LDLR. (C) Transcription factor analysis showing SREBF2 regulon activity score is increased in tumor-derived epithelial cells. (D) Western blot, where protein expression of ABCG1 is 1.7 times higher in murine bone marrow–derived monocytes treated with TCM compared with control condition with M-CSF. (E) Immunofluorescence of CD163 (green), ABCG1 (red), and DAPI (blue) in healthy human pancreas and PDA. (F) Immunofluorescence of LDLR (green), panCK (red), and DAPI (blue) in healthy human pancreas and PDA. Immunofluorescence from E and F is representative of 3 healthy individuals and 3 individuals with PDA, with staining performed twice per sample. For the low-magnification images, the scale bar is 50 μm, and for the zoomed insets, the scale bar is 25 μm.