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. 2024 Aug 15;9(18):e168889. doi: 10.1172/jci.insight.168889

Figure 4. Sfrp1 deficiency in fibroblast-derived EVs attenuates lung fibrosis in vivo.

Figure 4

(A) General outline of the experiment. EVs were isolated from the conditioned media of primary mouse lung fibroblasts (pmLFs) isolated from mice with genetic deletion of Sfrp1 (Sfrp1–/–) or WT control (Sfrp1+/+). SFRP1 expression was verified by Western blot (left panel). Isolated vesicles were characterized for size by NTA and injected intratracheally in mice previously challenged with bleomycin or control NaCl. E.O.D., every other day. (B) Representative histology of the lung of the above-described mice at D21 after bleomycin exposure. Scale bars indicate 2.5 μm or 100 μm (zoom). (C) Collagen quantification on FFPE lung sections stained with Picrosirius red and visualized under polarized light. Representative observation (n = 10 for BLM+Sfrp1+/+ pmLF-EVs and n = 9 BLM+Sfrp1–/– pmLF-EVs, left panel. Scale bar = 100 μm) and quantification (right panel) are shown. Statistical analysis by nonparametric Mann-Whitney. Each point represents 1 mouse. P values are indicated for each comparison.