Table 1.

Identification of spontaneous revertant mutations.

Chromosome	Position	DNA change	Protein change	Coding impact	Gene	Description from SGDa	Frequency
chrXIII	553608	247C>T	Nonsense at Q83	nonsense	FDO1 (YMR144W)	Protein involved in directionality of mating-type switching	1.00
chrIV	322263	38C>T	T13I	missense	RPL31A (YDL075W)	Ribosomal 60S subunit protein L31A	0.91
chrIV	178744	411G>T	W137C	missense	MSH5 (YDL154W)	Protein of the MutS family	0.59
chrVIII	19897	1075G>C	A359P	missense	ARN1 (YHL040C)	ARN family transporter for siderophore-iron chelates	0.73
chrIV	1193539	1346T>C	I449T	missense	EAF1 (YDR359C)	Component of the NuA4 histone acetyltransferase complex	0.24

^a Description of gene from SGD.