(A) Virus shedding from pigs intranasally infected with PrV-Ka, PrV-gD− Pass, or PrV gD− Pass SPH was analyzed by titration of nasal swabs during primary infection. On day 38 p.i., surviving animals and three untreated, age-matched piglets (control) were intranasally infected with 5 × 107 PFU of PrV NIA-3. (B) Virus shedding was again quantified by titration of nasal swabs. Average titers and standard deviation (error bars) are shown. □, WT; ●, gD− Pass SPH; ▵, gD− Pass; ×, control.