FIG. 7.

Transcomplementation of PrV-gD⁻ Pass gB⁻ (A) or PrV-gD⁻ Pass gH⁻ (B). (A) Either normal RK13 cells or RK13 cells expressing wild-type gB (gB WT), gB Pass, or wild-type gB and gD (gB/gD WT) were infected at an MOI of 0.1 with transcomplemented PrV-gB⁻ (top) or PrV-gD⁻ Pass gB⁻ (bottom). After complete CPE was observed, supernatants were harvested and titrated on gB Pass-expressing cells. (B) Normal Vero cells or Vero cells expressing wild-type gH (gH WT), gH Pass, or the gDH hybrid protein (19) were infected with transcomplemented PrV-gH⁻ (top) or PrV-gD⁻ Pass gH⁻ (bottom) at an MOI of 0.1. After complete CPE was observed, supernatants were harvested and titrated on gH Pass-expressing cells. Indicated are average titers of four independent experiments. Bars show standard deviations.