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Transcomplementation of PrV-gD−gH−. Normal Vero cells and Vero cells expressing gB and gH of wild-type PrV (gBgH WT), PrV-gD− Pass (gBgH Pass), or the gDH hybrid protein (gDH) were infected with gDH-transcomplemented PrV-gD−gH− (PrV-gDH−) at an MOI of 0.1. After the development of complete CPE, supernatants were harvested and titrated on gH Pass-expressing cells. The average titers of two independent experiments are shown. Bars show standard deviation.
