Figure 5.

Cd2ap is largely dispensable for cognitive and motor behaviors. Representative results are shown from behavioral assessments of Cd2ap conditional knockout animals (cKO; Cd2ap^f/f;Nes-Cre) and controls (CD2AP^f/f). Mice were examined at either 3.5 (A) or 12 months of age (B). Overall, no significant differences were detected (all P > 0.05), except for increased locomotor activity among 3.5-month-old Cd2ap conditional knockout mice during the first 10 min of the open field assay and decreased activity for 12-month-old Cd2ap cKO during the last 10 min of the open field assay. Statistical analysis was based on t-tests. For assessments of young mice, samples sizes of N = 23 cKO (13F and 10M) and 13 control (6F and 7M) mice were used for the elevated plus maze and hot plate tests; N = 24 cKO (14F and 10M) and 12 control (5F and 7M) mice for open field; and N = 19 cKO (with 10F and 9M) and 11 (with 5F and 6M) control mice were examined for novel object preference. For studies of aged mice, sample sizes were of N = 15 cKO (9F and 6M) and 22 control (10F and 12M) mice were used for the elevated plus maze tests; N = 15 cKO (with 9F and 6M) and 23 control (11F and 12M) mice for open field; N = 12 cKO (7F and 5M) and 16 control (7F and 9M) mice for novel object preference; and N = 16 cKO (9F and 7M) and 25 controls (10F and 15M) for hotplate testing. The color of individual data points indicates the sex of each animal, with teal indicating male mice and lavender indicating female mice. ns, non-significant; ^*P < 0.05; ^**P < 0.01. All error bars indicate mean ± SEM. See also Fig. S9 for results of additional assays.