Skip to main content
. Author manuscript; available in PMC: 2024 Oct 7.
Published in final edited form as: Biomaterials. 2022 May 25;286:121601. doi: 10.1016/j.biomaterials.2022.121601

Figure 5. FBS impairs biomaterial-directed bone repair outcomes.

Figure 5.

(a) Schematic of murine radial segmental defect model. PEG-4MAL hydrogels containing 50 ng BMP-2 and either RPMI 1640 + 3.2% MSC-qualified FBS (FBS) or PBS as the buffer component were cast inside 4 mm polyimide tubes. 2.5 mm unilateral critical-sized radial defects were created in C57BL/6J mice and the hydrogel-containing implant tube was introduced into the defect site. 4 weeks post-surgery, bone volume was analyzed by micro-computed tomography. (b) Representative 3D μCT reconstructions of week 4 defects. (c) μCT calculated bone volume within the middle 2 mm of the radial defect (n=9 over 2 independent experiments); mean ± SEM. Differences in bone volume were calculated by a two-tailed t-test.