FIG. 8.

Real-time PCR analysis of early (A) and late (B) viral transcripts in MDM. MDM were infected with equivalent amounts of DNase-treated virus stocks and analyzed by real-time PCR at 18 or 36 h after infection as described in Materials and Methods. Mock-infected cells were incubated with DNase-treated medium. ADA was heat inactivated by incubation at 56°C for 1 h. The results are expressed as copies of HIV-1 DNA per 10,000 copies of beta-globin. The data shown are representative of two independent experiments using cells obtained from different donors.