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. Author manuscript; available in PMC: 2024 Oct 8.
Published in final edited form as: Gut. 2024 Oct 7;73(11):1854–1869. doi: 10.1136/gutjnl-2023-331447

Fig 4. Alcohol binges promote neutrophil infiltration and neutrophil extracellular trap formation in MASH mice.

Fig 4.

(A) Representative Ly6G immunohistochemistry images from liver sections. Scale bar=50 μm (n=8 mice/group and average of 3–5 images per mice). (B) Quantification of percentage area of Ly6G+ positive cells using Image J. Whole-cell liver lysates were used to detect CXCL1(C), LCN2 (D), NE (G) and Cit-H3 (H) by ELISA. (n=3–8 mice/group). (E-F) Levels of LCN2 and NE in serum as measured by ELISA (n=3–5mice/group). (I-J) Co-immunofluorescence staining with NE and Histone H3 (I) and with LCN2 and Histone H3 (J) to visualize NETs production in mouse liver. scale bar=50 μm. Whole-cell human liver lysates were used to detect CXCL1(K), LCN2 (L), and NE (M) by ELISA. (n=6 patients/MASH, n=5 patients/AH (BMI≤25) and n=7 patients/AH (BMI≥25)). * p<0.05, **p<0.005, ***p<0.0005, ****p<0.0000