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. 2024 Oct 8;7:1278. doi: 10.1038/s42003-024-06986-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — a Histological comparative analysis of E13.5 mouse kidney and E30 pig kidney. Individual variation in fetal pig kidney size was observed, and representative examples of larger (E30 Pig-1) and smaller (E30 Pig-2) kidneys were presented. White arrowheads indicate RVs and yellow arrowheads indicate comma-shaped bodies or S-shaped bodies. Scale bars represent 200 μm. b Experimental scheme of organoid formation using fetal pig kidneys. c Bright-field images of pig kidney organoids formed under different culture conditions. Scale bar represents 200 μm. d Immunostaining images of pig kidney organoids at Day 6 that were stained by antibody against a distal tubule marker and a ureteric bud marker. Scale bar represents 200 μm. e Immunostaining images of pig kidney organoids at Day 6 that were stained by antibody against a glomerular marker and a ureteric bud marker. Scale bar represents 200 μm. f Quantitative analysis of cell composition ratio for fetal pig kidney organoids under various culture conditions.