Fig. 5.

Cellular distribution and quantification of myeloid, fibroblast-like and lymphoid cells in the granulomas and their surroundings. (A) Heat map showing the median of positive cell counts of the cellular markers per disease group (CVID, sarcoidosis, PS or TB), normalized towards the total cells detected in that region (granuloma or surrounding). B-D) 10x representative immunofluorescence single stains together with DAPI per IF multiplex assay performed, always showing the same biopsy sample per disease of all three IF assays, complemented with the quantified positive cell counts per total cells detected of the indicated regions and markers per disease group, with violin plots showing median and interquartile range; (B) DAPI = dark blue, CD68 = aqua, CD11c = yellow, PD-L1 = white, FAPα = red, SMA = green; (C) DAPI = dark blue, CD68 = aqua, CD163 = red, pERK1/2 = white, MPO = yellow, FAPa = green; (D) DAPI = dark blue, CD3 = red, CD4 = yellow, FOXP3 = aqua, CD20 = green, PD-1 = white. Statistical analysis with 2way anova, with Tukey’s multiple comparisons test, * = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.001