Table 6.

Collagenases used for cell separation from related tissues

Separated tissue	Cell	Enzyme	Concentration	Result	References
Adipose tissue	Adipose-derived stem cells (ADSCs)	Collagenase I	0.1%–0.2%	The most commonly used enzyme for separating adipose-derived stem cells.	[59,94]
		MMP-12 (recombinant expression)	/	The ADSCs obtained exhibited remarkably similar morphological and phenotypical characteristics as cells isolated using collagenase I.	[95]
Pancreatic tissue	Islet cells	Collagenase V	0.5–2.5 g/ml	The islet cells were isolated effectively, but the use of collagenase alone resulted in low islet production.	[96]
		Liberase HI (collagenase I, II, and thermolysin enzyme)	According to the instructions	The most widely used commercially available enzyme for clinical islet isolation.	[60]
		Collagenase NB1/NP	According to the instructions	Compared to liberase HI, the volume of digestive tissue is reduced and the number of islets obtained is lower.	[97]
Liver	Hepatocytes	Collagenase VI	/	The protection of surface protein activity, especially mGluR5, was obviously enhanced in isolated rat hepatocytes.	[98]
		N-Acetylcysteine and liberase	According to the instructions	The efficiency of hepatocyte separation was obviously improved, and the production of living cells was increased.	[99,100]
		Collagenase V	0.5 mg/ml	It exhibits comparable efficacy to the liberase enzyme while providing superior cell viability.	[100]