Fig. 2.

Analysis of VIM and SEPT9 expression in CK19‐positive iCCA tumor cells. (A) Subset procedure to analyze tumor cells of iCCA origin in the single‐cell transcriptome in liver cancer samples. (B) t‐SNE DimPlot resulting in a subset of 839 iCCA tumor cells comprising the following cell phenotypes: malignant cells and HPC‐like. (C) t‐SNE DimPlot of the CK19 expression subset of iCCA tumor cells stratified by their phenotype. (D, F) t‐SNE DimPlot of SEPT9/VIM expression in iCCA tumor cells stratified by their phenotype. (E, G) Scatterplot of CK19 versus SEPT9/VIM expression in iCCA tumor cells. (CK19, cytokeratin 19; iCCA, Intrahepatic cholangiocarcinoma; SEPT9, septin 9 gene; t‐SNE, t‐distributed Stochastic Neighbor Embedding; VIM, vimentin gene).