Table 2.

Number and size of spontaneous metastasis in orthotopic xenograft mice assay. Metastatic lesions from orthotopic tumors were visualized at necropsy and scored by gross examination under a Leica ST microscope. The number of metastases in the lungs and liver per individual mouse was recorded, and the major diameter of the lesions was measured. Means ± SD are shown calculated for all mice included in each group. The number of mice and day of sacrifice are indicated in Table 1.

Number and size of spontaneous metastasis in NSG mice
	Lung		Liver
	Number of metastases /mice	Ø (mm) of metastases	Number of metastases/mice	Ø (mm) of metastases
SK‐Mel 131 mock	5.5 ± 0.5	1.2 ± 0	2 ± 0	2.35 ± 0.39
Mel 131 sh PRRX1	6.5 ± 1.323	1.94 ± 0.48	1 ± 0	1.43 ± 0.03
WM 793 mock	2.75 ± 0.75	0.84 ± 0.07	4 ± 0.75	3.3 ± 0.9
793 sh PRRX1	19.3 ± 2.1	1.4 ± 0.1	8 ± 3.7	2.2 ± 0.44
A375 MM mock	10.5 ± 0.5	3 ± 0	13 ± 1.5	4.65 ± 0.65
375 MM sh PRRX1	22.2 ± 5.6	1.8 ± 0.39	17.2 ± 7.4	4.24 ± 0.44