FIG. 7.
Effects of PKR inactivation on TNF-α induction by HSV. (A) RAW-pBK-CMV and RAW-PKR-M7 cells were treated with 100 IU of IFN-γ per ml and infected with 3 × 105 PFU (multiplicity of infection [MOI], 0.6) of HSV-2 per ml as indicated. (B) The cells were treated as for panel A with the sole modification that the virus was UV irradiated prior to addition to the cell cultures. (C) RAW-PKR-M7 cells were treated with 100 IU of IFN-γ per ml and 3 × 105 PFU (MOI, 0.6) of gL86/79VB4 per ml or equivalent amounts of gL86/Vero. (A to C) After 8 h of infection the levels of bioactive TNF-α in the supernatants were assayed. Results are shown as means of duplicate cultures ± standard errors of the means. Similar results were obtained in three independent experiments. ∗, P < 0.05.