FIG. 7.

Seven of seven SIV- or SHIV-infected Mamu-A*02⁺ rhesus monkeys demonstrate CTL recognition of SIV Nef p199RY (YTSGPGIRY). PBL from seven rhesus monkeys, as indicated, were stimulated in vitro with 5 μg/ml SIV Nef p199RY. On days 13 and 14 of culture, the lymphocytes were assessed as effector cells in a standard 4-h ⁵¹Cr release CTL assay. Target cells were B-LCL of monkey 24195 (Mamu-A*01⁻ Mamu-A*02⁺) that had been incubated overnight in 5 μg/ml of either SIV Nef p199RY or SIV Nef p199S as a negative control. For effectors from monkeys 833 and 483, B-LCL from monkey 19196 (Mamu-A*01⁺ Mamu-A*02⁺) were used as targets, treated as above. Effectors were added at a 2:1 E:T ratio.