Figure 3.

ASCL1, NEUROD1, POU2F3, and YAP1 gene expression profile of SCLC. (A) RNA expression levels of ASCL1, NEUROD1, POU2F3, and YAP1 genes in the tested 35 tumor samples by RNA STEP. RNA STEP results of samples 1 to 31 and 32 to 33 were ordered by highest to lowest ASCL1 and NEUROD1 results, respectively. (B, C) Comparison of ASCL1, NEUROD1, POU2F3, and YAP1 gene expression levels in primary and metastatic tumors of same patient (#32, 54 y old, male) by RNA STEP. The distinct expression profiles observed in primary and metastatic tissues highlight substantial intrapatient heterogeneity. (B) Visual representation illustrating the gene expression profile of each tumor site. Created using BioRender.com. (C) Gene expression levels assessed by RNA STEP. RNA STEP, RNA Salah Targeted Expression Panel.