TABLE 1.
Primer sets used for RT-PCR and restriction endonucleases used to prepare PCR products for SSCP analysis
| Primer set | Downstream primera | Upstream primera | Restriction endonuclease |
|---|---|---|---|
| RCF1 | AGCTGGATCCTTTTTTTTTTTTTT | ||
| P1 | CCGGATCCCTAATAAGTTGTGATAAAGCC | CCGGATCCCAATGGCAACAGCGAATTGT | Sau3AI |
| HC-Pro | CCGAATCCTCAGCCAATTTTGTAATCTTT | CCGGATCCCCAGTGGCATACAATAC | HaeIII-NheI |
| P3 | CCGGATCCTATTGGTATTCAACCAATTC | GCGGATCCGCGGGTTCCAAGAGACTGTT | RsaI |
| CI5′ | CAACGACAACGTCTGCATGCAAATTCAC | GTACCCGGGGAAGCGAGAGGAAG | Sau3AI |
| CI3′ | GAGGATCCTCACTCGAACTTGACACTACG | GATGTCGAAGCACTCCGAGCTGAGAAG | HaeIII |
| NIa | GACTTCTAGATCATTGCCAACTAACCAAG | GTCTAAGCTTGGGCAAAGCAGCACGCA | TaqI |
| NIb | GGGGATCCTCATTCGTACACGCAGTATTG | CCGGATCCCCAGTCTCCAAATGACGAAA | AluI |
| CP | CGATTTTTTTTTTTTTTTGCCGTCGCCCT | AGCTTATGAGCGCTATTATTGCAGCAT | AluI |
a
Underlines denote 5′-terminal regions of primers that do not correspond to WSMV sequence.