TABLE 4.

Sporadic nucleotide changes in clones derived from LDSIs of WSMV Sidney 81 and Type

Strain	PCR product	No. of sporadic changes/nt sequenced (10 clones each)	No. of sporadic changes/nt/cycle of RT-PCR
Sidney 81	P1	4/8,160	0.14 × 10−4
Type	P1	8/8,160	0.27 × 10−4
Sidney 81	P3	5/7,980	0.17 × 10−4
Type	P3	7/7,980	0.24 × 10−4
Sidney 81	NIa	14/12,460	0.36 × 10−4
Type	NIa	16/12,460	0.41 × 10−4
Sidney 81	CP	13/13,710	0.26 × 10−4
Type	CP	12/13,710	0.24 × 10−4
Totals		79a/84,620	0.27 × 10−4b
NIa reclone		15/12,460	0.40 × 10−4

^a69 transitions, 9 transversions, and 1 single-base deletion. 

^bWeighted average.