TABLE 6.

Nucleotide substitutions detected among lineages passaged from LDSI-S10

PCR product	Nucleotide substitutiona at:				Amino acid change
	Passage 1	Passage 3	Passage 6	Passage 9
Wheat lineage WA
NIa	X	X	G6016→A	G6016→A	Ser→Ser
	X	X	G*5743→A*	G5743→A	Met→Ile
	X	X	G*6298→A*	G6298→A	Arg→Arg
CP	X	X	U*8194→C*	U8194→C	Ser→Ser
	X	X	A8964→G	A8964→G	Lys→Arg
Wheat lineage WB
NIa	X	X	G6016→A	G6016→A	Ser→Ser
	X	X	U*6418→C*	X	His→His
CP	X	X	X	C8556→U	Ala→Val
	X	X	C*8764→U*	C*8764→U*	Cys→Cys
	X	X	A*8765→C*	A*8765→C*	Ile→Leu
Wheat lineage WC
NIa	X	X	G6016→A	G6016→A	Ser→Ser
CP	X	X	A8248→U	A*8248→U*	Ala→Ala
	X	X	C8764→U	C*8764→U*	Cys→Cys
	X	X	A8765→C	A*8765→C*	Ile→Leu
Corn lineage CA
NIa	X	X	G6016→A	G6016→A	Ser→Ser
CP	X	X	G8423→A*	G8423→A	Asn→Asn
			C9259→U	C9259→U	3′-UTR
Corn lineage CB
P1	C325→U	C325→U	C325→U	C325→U	Asn→Asn
NIa	X	X	G6016→A	G6016→A	Ser→Ser
	X	X	X	G6127→A	Met→Ile
NIb	X	X	X	C7318→U	Phe→Phe
CP	X	X	X	C8880→U	Ala→Val
Corn lineage CC
P1	X	X	U961→C	X	Phe→Phe
CI	C3466→U	X	C3466→U	C3466 →U	Ile→Ile
NIa	U6418→C	U6418→C	U6418→C	U6418→C	His→His
NIb	X	X	C7621→U	C7621→U	Gly→Gly
Barley lineage BA
NIa	X	X	G6016→A	G6016→A	Ser→Ser
NIb	X	X	C8014→U	X	Phe→Phe
CP	X	X	X	A8502→T	Gly→Gly
Barley lineage BB
CI	X	A4384→G	A4384→G	A4384→G	Ser→Ser
NIa	X	X	U5668→C	U5668→C	Gln→Leu
	X	X	G6016→A	G6016→A	Ser→Ser
Barley lineage BC
NIa	X	X	G6016→A	G6016→A	Ser→Ser

^aX, no change from LDSI-S10; *, both nucleotides detected in the same lineage and passage.