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Structure of the RhPV genome and plasmids used in this study. Various fragments of the 5′ end of the RhPV genome were amplified by PCR using primers containing BamHI sites, digested, and inserted between the CAT and LUC ORFs (at the unique BamHI site) in plasmid pGEM-CAT/LUC as described in Materials and Methods. Nucleotide numbers corresponding to the fragments are shown. AUG codons within the RhPV 5′ UTR are indicated by arrowheads. The IGR of RhPV is also indicated.
