FIG. 1.

Schematic representation of the BamHI H region of the KOS genome. The BamHI H region of KOS (7.5 kb) contains genes UL37 and UL38 (22), with the latter being the gene for the capsid protein VP19C. The UL37 and UL38 ORFs specify 1,123 and 465 amino acid residues, respectively. Two cell lines expressing UL37 in trans were constructed. 3B13 and BD45 were transformed with the BamHI H and BamHI-to-DraI fragments, respectively. The virus designated KΔ19C contains a deletion in the UL38 gene which eliminates residues 1 to 415 of VP19C (29). Plasmid pKΔUL37 was constructed by deletion of sequences between the NotI restriction enzyme site at the 5′ end and the SpeI site at the 3′ end of UL37. This resulted in the deletion of residues 86 to 1120. The relevant restriction enzyme sites and the genome nucleotide numbers in parentheses are shown at the top of the figure.