FIG. 10.
Association of KΔUL37 DNA-filled capsids with the nuclear fraction of infected cells. Vero cells (2 × 107 cells in 100-mm-diameter dishes) were infected with KOS, KΔUL37, and KΔUL36 at an MOI of 10 PFU/cell. Infected cells were metabolically labeled with [3H]thymidine from 8 to 24 h after infection. Infected cells were fractioned into crude cytoplasmic (CYTO) and nuclear (NUC) fractions and the lysates were sedimented through 20 to 50% sucrose gradients. The total radioactivity present in the peak fractions, which contained C capsids, from each gradient is plotted as a percentage of the total radioactivity, that is, the sum of the CYTO and NUC radioactivity. Error bars indicate standard deviations of the data.