FIG. 3.

Western blot analysis of KΔUL37-infected cells. Vero (lanes 1 and 2), 3B13 (lanes 3 and 4), and BD45 (lanes 5 and 6) cells were infected at an MOI of 10 PFU/cell with KOS (lanes 1, 3, and 5) or KΔUL37 (lanes 2, 4, and 6). Infected cells were harvested 24 h after infection and protein extracts were prepared. Total infected cell polypeptides were resolved by SDS-PAGE (12% acrylamide) and transferred onto a membrane. The proteins on the filter were incubated with UL37 antisera (708; see reference 2) and hybridization was monitored by ¹²⁵I-labeled protein A.