FIG. 5.

Capsid formation in KΔUL37-infected cells. Vero cell monolayers (10⁷ cells in 100-mm-diameter dishes) were infected with KOS (A) and KΔUL37 (B) at an MOI of 10 PFU/cell and labeled with [³⁵S]methionine from 8 to 24 h postinfection. Nuclear extracts were prepared and layered onto 20 to 50% sucrose gradients. Fractions collected after sedimentation were analyzed by SDS-PAGE (17% acrylamide). Direction of sedimentation was from right to left. The positions of capsid proteins are indicated on the right of the figure for KOS. The positions at which A, B, and C capsids sediment are indicated below the figure.