Fig. 4.

Invasive metastasis assay of non-small cell lung cancer cells (LLC) under different conditioned media. A, B The migration ability of LLC cells under different M2-like macrophages conditioned media was detected by transwell migration assays (scale bar = 100 µm). C, D Cell invasion ability of LLC cells under different M2-like macrophage-conditioned media was detected by transwell invasion assays (scale bar = 100 µm). E, F Apoptosis assay of LLC cells under different M2-like macrophage conditioned media. G, H Colony-forming assays of LLC cells under different M2-like macrophage-conditioned media. Data was presented by the mean ± SD (n = 3) per group. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001