Table 3.
Prevalence of Blastocystis in Portuguese wild boars (n = 99) according to the bioregion of origin and sampling year.
| Variable | Samples (n) | Blastocystis positive (n)a | Blastocystis positive (%) | 95% CI (%) | P value | Subtypes detectedb (n) |
|---|---|---|---|---|---|---|
| Bioregion | 0.515 | |||||
| BR1 | 12 | 3 | 25.0 | 5.5–57.2 | ST5 (3) | |
| BR2 | 39 | 9 | 23.1 | 11.1–39.3 | ST5 (6), ST5/ST10a (1), ST5/ST14 (1), ST5/ST43 (1) | |
| BR3 | 48 | 22 | 45.8 | 31.4–60.8 | ST5 (18), ST5/ST13 (1), ST5/ST15 (1), ST5/ST24b (1), ST5/ST43 (1) | |
| Sampling year | 0.373 | |||||
| 2019 | 64 | 18 | 28.1 | 17.6–40.8 | ST5 (14), ST5/ST10a (1), ST5/ST14 (1), ST5/ST24b (1), ST5/ST43 (2) | |
| 2020 | 21 | 8 | 38.1 | 18.1–61.6 | ST5 (8) | |
| 2021 | 14 | 8 | 57.1 | 28.9–82.3 | ST5 (6), ST5/ST13 (1), ST5/ST15 (1) |
All the samples were collected from naturally classified areas. 95% confidence intervals (95% CI) are included. BR1 encompasses Central Portugal West (CPW), BR2 Montesinho Natural Park (MNP), and BR3 Central Portugal East (CPE) and Malcata Nature Reserve (MNR). Subtypes previously reported in humans (regardless of their true zoonotic potential) are in bold.
aSamples were considered positive when Blastocystis was identified after Sanger and next-generation sequencing.
bSubtype information obtained from all positive samples via next-generation amplicon sequencing.