Table 4.
Diversity of Blastocystis subtypes and unique genetic variants observed using next-generation amplicon sequencing (NGS) among Blastocystis-positive wild boars from Spain (n = 22) and Portugal (n = 34).
| Subtype | Subgroup | Samples (n) | Unique genetic variants (n) | Frequency of positive simples (%) | GenBank accession number(s)a |
|---|---|---|---|---|---|
| ST5 | 56 | 8 | 100 | OR730909(P)/OR730910(S) | |
| OR730911(P)/OR730912(S) | |||||
| OR730916(P)/OR730917(S) | |||||
| OR730918(P)/OR730919(S) | |||||
| OR730933(S) | |||||
| OR730938(S) | |||||
| OR730943(S) | |||||
| OR730947(P) | |||||
| ST10 | ST10a | 1 | 1 | 1.8 | OR730914(P) |
| ST13 | 1 | 1 | 1.8 | OR730913(P) | |
| ST14 | 1 | 1 | 1.8 | OR730946(P) | |
| ST15 | 2 | 2 | 3.6 | OR730944(S) | |
| OR730945(P) | |||||
| ST24 | ST24b | 1 | 1 | 1.8 | OR730915(P) |
| ST43 | 2 | 1 | 3.6 | OR730924(P) |
Subtypes previously reported in humans (regardless of their true zoonotic potential) are in bold.
a For unique variants identified in Spain and Portugal, two sequences were submitted to GenBank. The country in which the sequences were identified is denoted in parentheses by the GenBank accession number. P and S denote Portugal and Spain, respectively.