TABLE 1.
Oligonucleotide primers used for PCR amplification and sequencing of human polyomaviruses BKV and JCV
| Virus type (region) | Position | Primer | Sequencea |
|---|---|---|---|
| JCV (IGR) | 2062–2087b | EP1A | 5′-TGAATGTTGGGTTCCTGATCCCACC-3′ |
| JCV (IGR) | 2774–2798 | EP2A | 5′-ACCCATTCTTGACTTTCCTAGAGAG-3′ |
| JCV (IGR) | 2099–2124 | P1Ad | 5′-CAAGATATTTTGGGACACTAACAGG-3′ |
| JCV (IGR) | 2742–2766 | P2Ad | 5′-CCATGTCCAGAGTCTTCTGCTTCAG-3′ |
| JCV (IGR) | 2511–2536 | JCSR | 5′-TGATTACAGCATTTTTGTCTGCAAC-3′ |
| JCV (IGR) | 2364–2388 | JCSL | 5′-GGAAGTCCTTCTGTTAATTAAATCAG-3′ |
| JCV (RR) | 4992–5011 | JR1e | 5′-CCCTATTCAGCACTTTGTCC-3′ |
| JCV (RR) | 428–447 | JR2e | 5′-CAAACCACTGTGTCTCTGTC-3′ |
| JCV (RR) | 5060–5079 | JR3e | 5′-GGGAATTTCCCTGGCCTCCT-3′ |
| JCV (RR) | 298–317 | JR4e | 5′-ACTTTCACAGAAGCCTTACG-3′ |
| BKV (VP1) | 1452–1467c | BK1 | 5′-TATTGCCCCAGGAGGT-3′ |
| BKV (VP1) | 2132–2148 | BK2 | 5′-AACATTTTCCCCTCCTG-3′ |
| BKV (VP1) | 1762–1781 | BK4 | 5′-AGTAGATTTCCACAGGTTAG-3′ |
| BKV (VP1) | 1486–1506 | BK6 | 5′-CCAGGGGCAGCTCCCAAAAAG-3′ |
| BKV (VP1) | 1425–1442 | BK3 | 5′-ACTGTAACACCTGCTCTT-3′ |
| BKV (RR) | 5024–5043 | BR1 | 5′-CCCTGTTWARRACTTTATCC-3′ |
| BKV (RR) | 431–457 | BR2 | 5′-GTAAAGCAGTGGTACTTT-3′ |
| BKV (RR) | 5083–5102 | BR3 | 5′-ATAGTTTTGCTAGGCCTCAG-3′ |
| BKV (RR) | 305–322 | BR4 | 5′-CAACTTTCACTGAAGCTT-3′ |