Fig 6. Akt phosphorylation at both Ser⁴⁷³ and Thr³⁰⁸ is required for IE1 induction in monocytes infected with US28Δ HCMV.

(A to D) Monocytes were transfected with scrambled (NC), PDK1, or rictor siRNA for 48 h and infected with WT or US28Δ HCMV for 30 min (A, B) or 24 h (C, D). PDK1, rictor, total Akt, phosphorylation of Akt at Ser⁴⁷³, phosphorylation of Akt at Thr³⁰⁸, and IE1 were determined by Western blot (A and B) and quantified (C and D). β-actin was used as a loading control. Western blots and densitometry are representative of at least 3 biological replicates per group. **P < 0.005, ***P < 0.0005, **** P < 0.0001 by one-way ANOVA with Tukey’s HSD post hoc test.