Fig 7. US28 does not regulate SHIP1 activity.

(A to G) Monocytes were mock-infected or infected with WT or US28Δ HCMV (MOI = 1) for 30 min and incubated with ethanol (EtOH; carrier control) or 3AC (5 μM; SHIP1 inhibitor) for 30 min (A to C) or 24 h (D to F). Total SHIP1, p-SHIP1, total Akt, phosphorylation of Akt at Ser⁴⁷³, and phosphorylation of Akt at Thr³⁰⁸ were detected by Western blot (A to D). IE1 protein and UL123 transcript levels were detected by Western blot (E, F) or RT-qPCR analysis (G), respectively. Western blots and densitometry are representative of at least 3 biological replicates per group. RT-qPCR data are means ± SEM from at least 3 biological replicates per group. ns= not significant, *P<0.05, ***P < 0.0005, ****, P < 0.0001 by one-way ANOVA with Tukey’s HSD post hoc test.