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Dot blot assay for YLD virus DNA in infected cell lines over time. CHO, RK13: MC38, 1299, and CV-1 cells were infected at an MOI of 5 with YLD virus, and cell lysates were probed with 32P-labeled TK cDNA. Only 1299 and CV-1 cells demonstrate DNA amplification consistent with viral replication. More-efficient DNA synthesis occurs in CV-1 cells than in 1299 cells.
