FIG. 4.

Western blot analysis of the gD expressed in F-US6kan-infected cells. HaCaT cells were infected with wild-type HSV-1 F or noncomplemented F-US6kan using 5 PFU/cell (multiplicity of infection [MOI]) or with wild-type HSV-1 using 0.005 PFU/cell or were left uninfected. After 9 h, the cells were lysed with detergent, and extracts (either 5 or 25 μl) were subjected to electrophoresis and then transferred to Immobilon P membranes that were probed with anti-gD MAb ID3. (A) Blot exposed to film for 5 s; (B) the same blot exposed for 30 s.