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. 2024 Sep 10;27(10):110919. doi: 10.1016/j.isci.2024.110919

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© 2024 Published by Elsevier Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Aging and Arctic expression have differential effects on the axon terminals and active zone scaffold of Gr64f neurons

(A) Expression pattern of Gr64f in 10-day-old and 40-day-old flies is visualized with GFP (green). ROIs indicating the labellar (yellow) and the taste peg/internal mouthpart projections (blue) are shown. Scale bar: 50 μm.

(B) There is a significant effect of age on Gr64f neurite volume (two-way repeated measures ANOVA: F_1,26 = 23.29, p < 0.0001; n = 12–16).

(C) There is a significant effect of age on Gr64f neurite intensity (two-way repeated measures ANOVA: F_1,26 = 167.5, p < 0.0001; n = 12–16).

(D) Expression pattern of Gr64f in control and AD model flies is visualized with GFP (green).

(E) There is a significant effect of Arctic expression on Gr64f neurite volume (two-way repeated measures ANOVA: F_1,24 = 22.98, p < 0.0001; n = 13).

(F) There is a significant effect of Arctic expression on Gr64f neurite intensity (two-way repeated measures ANOVA: F_1,24 = 45.01, p < 0.0001; n = 13).

(G) The active zone of Gr64f neurons in young and aged flies is visualized with Brp-Short (red). ROIs indicating the labellar (yellow) and the taste peg/internal mouthpart projections (blue) are shown. Scale bar: 50 μm.

(H) There is a significant effect of age on Brp volume (two-way repeated measures ANOVA: F_1,28 = 26.18, p < 0.0001; n = 13–17).

(I) There is a significant effect of age on Brp intensity (two-way repeated measures ANOVA: F_1,28 = 43.09, p < 0.0001; n = 13–17).

(J) The active zone of Gr64f neurons in control and AD model flies is visualized with Brp-Short (red).

(K) There is a significant effect of Arctic expression on Brp volume (two-way repeated measures ANOVA: F_1,29 = 8.244, p < 0.0076; n = 15–16).

(L) There is no effect of Arctic expression on Brp intensity (two-way repeated measures ANOVA: F_1,29 = 1.706, p < 0.2018; n = 15–16). The median (solid line) as well as 25th and 75th percentiles (dotted lines) are shown. a.u. = arbitrary units. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.