Figure 4.

snRNA-sequencing of the labellum during aging and in AD model flies

(A) Schematic of snRNA-sequencing workflow. The labellum of adult flies were dissected and dissociated to obtain single nuclei for 10× Genomics snRNA-seq. Error bars indicate ±SEM. FACS: florescence-activated cell sorting.

(B) Number of nuclei collected from each sample.

(C) Uniform Manifold Approximation and Projection (UMAP) visualization.

(D) UMAP visualization representing unique clusters. Each color and dot on the plot represent a unique cluster and nucleus, respectively.

(E) The proportion of nuclei for each broad cell cluster shown in (D).

(F) UMAP visualization representing the average expression of Gr64f+ nuclei.

(G) Volcano plot depicting differentially expressed genes within the Gr64f+ nuclei between 10-day-old and 40-day-old flies.

(H) Volcano plot depicting differentially expressed genes within the Gr64f+ nuclei between control and Arctic-expressing flies. Genotypes: 10d: w¹¹¹⁸; 40d: w¹¹¹⁸; control: Gr64f-Gal4/+; Arctic: Gr64f-GAL4/UAS-Arctic.