Aging is associated with a modality-specific decline in taste

. 2024 Sep 10;27(10):110919. doi: 10.1016/j.isci.2024.110919

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies

Mouse monoclonal anti-bruchpilot (nc82-s)	Developmental Studies Hybridoma Bank	nc82 RRID: AB_2314866
Donkey anti-mouse Alexa Fluor 647	Invitrogen	A-31571

Chemicals, peptides, and recombinant proteins

Bloomington Formulation Nutri-fly food	Genesee Scientific	66–113
Hexanoic Acid	Sigma Aldrich	21530
Octanoic Acid	Sigma Aldrich	O3907
L-serine	Sigma Aldrich	84959
L-phenylalanine	Sigma Aldrich	P5482
Citric Acid	Sigma Aldrich	251275
Acetic Acid	Sigma Aldrich	695092
Sucrose	Fisher Scientific	FS S5-500
Fructose	Fisher Scientific	57-48-7
PBS	Fisher Scientific	BP243820
Paraformaldehyde	Sigma Aldrich	158127
Triton X-100	Sigma Aldrich	T8787
VECTASHIELD Antifade Mounting Medium	Vector Labs	H1000
NaCl	Sigma Aldrich	S9888
KCl	Sigma Aldrich	P9541
MgCl2	Sigma Aldrich	208337
CaCl2	Sigma Aldrich	C4901
HEPES-NaOH	Fisher Scientific	50-197-1

Deposited data

Raw behavioral and imaging data	This paper	Data S1
Raw and analyzed sequence data	This paper	GSE273715
The script used for Seurat analysis	This paper	Data S2

Experimental models: organisms/strains

w¹¹¹⁸ fly strain	Bloomington Drosophila Stock Center (BDSC)	5905
Gr64f-GAL4	BDSC	57668
UAS-mCD8:GFP	BDSC	32186
UAS-Aβ_1-40	BDSC	64215
UAS-Arctic	Crowther et al.²¹	–
UAS-GCaMP-R	Cho et al.⁴⁰	–
UAS-Brp-Short	Mosca and Luo⁴⁵	–

Software and algorithms

ImageJ/FIJI	Schindelin et al.⁷⁷	v1.54j
Nikon AR Image Analysis software	Nikon	v5.20.02
Microsoft Office 365	Microsoft	v2406
Prism	Graphpad software	v10.2.3
Python	https://www.anaconda.com/products/individual	v3.8.0
R	https://www.r-project.org/	v4.3
RStudio	https://www.rstudio.com/products/rstudio/download/	v3.6.0
Cell Ranger	10 $\times$ Genomics	v6.0
Seurat	Hao et al.⁷⁸	v4.3.0
Cell Marker Enrichment tool (DRscDB)	Hu et al.⁷⁹	–
clusterProfiler	Wu et al.⁸⁰	v4.8.3

Other

Incubators	Percival	I36-VL
Laser-scanning confocal microscope	Nikon	A1R
Micromanipulator	Narishige International USA	MM-3
Magnetic Stand	Narishige International USA	GJ-8
Capillary Tubes	World Precision Instruments	1B120F-4
Dental Glue	Ivoclar Vivadent	595953WW