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Autoradiographic image of in vitro-transcribed/translated UL42 followed by GST-cdc2dn pull down. Full-length (FL) or amino (N′) or carboxyl (C′) halves of UL42 protein were in vitro transcribed and translated (lanes 1 to 6). The in vitro-translated UL42 proteins were then reacted with GST or GST–cdc2-dn for pull down assays (lanes 7 to 12), electrophoretically separated, and developed by autoradiography.
