FIG. 2.
cdc2 immunoblot of HEp-2 cell lysates reacted with GST or GST fusion proteins expressing full-length (FL) or amino (N′) or carboxyl (C′) halves of UL42 protein. Immunoreactivity of cdc2 from whole-cell lysates is shown in lanes 1 and 2. Whole-cell lysates were then incubated with 2, 5, or 10 μl of GST (lanes 3 to 5), GST-UL42 FL (lanes 6 to 8), GST-UL42 N′ (lanes 9 to 11), or GST-UL42 C′ (lanes 12 to 14), and GST beads were collected, electrophoretically separated, and immunoblotted for cdc2.