FIG. 5.
Autoradiographic image of histone H1 kinase activity associated with UL42 immunocomplexes following immunodepletion. HSV-1-infected HEp2 cell lysates were immunodepleted with preimmune serum (lane 1), antibody (Ab) to cdc2 (lane 2), or antibody to UL42 (lane 3). The immunodepleted lysates were then immunprecipitated with antibody to UL42. Immunocomplexes were assayed for histone H1 kinase activity. Phosphorylated histone H1 was resolved by polyacrylamide gel electrophoresis, and the blots were developed by autoradiography. Histone phosphorylation was quantified by PhosphorImager analysis. The sample immunodepleted with preimmune serume was given a value of 1.