FIG. 6.
Autoradiographic image of histone H1 kinase activity from UL42 transfected cells. HEp-2 cells were transfected with pCDNA3.1 encoding UL42 (lanes 2 and 4). Cells were also treated with nocodazole following transfection (lanes 3 and 4). Cell lysates were then immunoprecipitated with antibody to UL42 and subjected to histone H1 kinase assays. Phosphorylated histone H1 was electrophoretically separated in a denaturing polyacrylamide gel and subjected to autoradiography. Histone phosphorylation was quantified by PhosphorImager analysis. Untransfected lysate without nocodazole treatment was assigned a value of 1.