Table 1. SARS-CoV-2 Multiplex Assay Reagents.
List of the primer and probe names and sequences as used in the SARS-CoV-2 multiplex assay.
| Primer/probe Name | Gene | Sequence (5′ to 3′) | Reporter Dye | Quencher | Final Concentration (nM) |
|---|---|---|---|---|---|
| 2019-nCoV_N1-FWD | SARS-CoV-2 N | GACCCCAAAATCAGCGAAAT | ABY | QSY | 500 |
| 2019-nCoV_N1-REV | TCTGGTTACTGCCAGTTGAATCTG | 500 | |||
| 2019-nCoV_N1-ABY | ACCCCGCATTACGTTTGGTGGACC | 250 | |||
| 2019-nCoV_N2-FWD | SARS-CoV-2 N | TTACAAACATTGGCCGCAAA | FAM | QSY | 500 |
| 2019-nCoV_N2-REV | GCGCGACATTCCGAAGAA | 500 | |||
| 2019-nCoV_N2-FAM | ACAATTTGCCCCCAGCGCTTCAG | 250 | |||
| RP-FWD | RPP30 | AGATTTGGACCTGCGAGCG | VIC | QSY | 100 |
| RP-REV | GAGCGGCTGTCTCCACAAGT | 100 | |||
| RP-VIC | TTCTGACCTGAAGGCTCTGCGCG | 50 |