Figure 3.

The comparison of (A) cleaved caspase 3/caspase 3, (B) Bax/Bcl2, (C) pBcl2/β-actin, and (D) BECN1/β-actin between in the control group and ciprofloxacin group. A concentration of 100 µg/mL of ciprofloxacin was used in comparison with control group. β-actin was used for the control. The examination was done under normoxia condition (21% O₂). Proteins were detected by western blot analysis. Cell viability was checked at 0, 6, 12, 24, and 48 hours after treatments. All data were performed more than 3 times in order to assess reproducibility and reliability of the results. Bax = Bcl-2-associated X protein, B-cell lymphoma 2.