Table 1.
STP‐B IC50 values in HMCLs and isogenic clones.
| HMCLs | TP53 sequence | p53 WB | Cytogenetic group | STP‐B IC50 (nM) |
|---|---|---|---|---|
| TP53 wt | ||||
| AMO1 | +/+ | + (53 kDa) | OTHER | 285 |
| MDN | +/+ | + (53 kDa) | CCND1 | 24 |
| MM1S | +/+ | + (53 kDa) | MAF | 3287 |
| NAN11 | +/− | + (53 kDa) | MAF | >10,000 |
| NCI‐H929 | +/+ | + (53 kDa) | MS | 245 |
| XG6 | +/+ | + (53 kDa) | MAF | 1937 |
| XG7 | +/+ | + (53 kDa) | MS | 356 |
| TP53Abn | ||||
| JIM3 | R273C/− | + (53 kDa) | MS | 2181 |
| JJN3 | −/− | ‐ | MAF | ND |
| KMM1 | C135F/S241F | + (53 kDa) | OTHER | 424 |
| KMS12PE | R337L/− | + (53 kDa) | CCND1 | >10,000 |
| L363 | S261T/− | +/− (53 kDa) | MAF | 440 |
| LP1 | E286K/− | + (53 kDa) | MS | ND |
| NAN1 | E180STOP/− | ‐ | MAF | 5031 |
| NAN3 | R248Q/− | + (53 kDa) | MS | 1966 |
| NAN6 | Del exons 7–9/− | + (35 kDa) | MAF | 322 |
| NAN8 | D21Y/− | ‐ | MS | 466 |
| OPM2 | R175H/− | + (53 kDa) | MS | ND |
| U266 | A161T/− | + (53 kDa) | CCND1 | ND |
| XG2 | C176Y/R213STOP | + (53 kDa) | OTHER | 533 |
| XG5 | R282W/− | + (53 kDa) | CCND1 | >10,000 |
| XG11 | C135Y/− | + (53 kDa) | CCND1 | 17 |
| TP53 clones | ||||
| NCI‐H929 TP53 +/+ #1 | +/+ | + (53 kDa) | MS | 165 |
| NCI‐H929 TP53 +/+ #2 | +/+ | + (53 kDa) | MS | 93 |
| NCI‐H929 TP53 −/− #1 | −/− | ‐ | MS | 1043 |
| NCI‐H929 TP53 −/− #2 | −/− | ‐ | MS | 486 |
| XG7 TP53 +/+ #1 | +/+ | + (53 kDa) | MS | 552 |
| XG7 TP53 +/+ #2 | +/+ | + (53 kDa) | MS | 379 |
| XG7 TP53 +/+ #3 | +/+ | + (53 kDa) | MS | 1108 |
| XG7 TP53 −/− #1 | −/− | ‐ | MS | 129 |
| XG7 TP53 −/− #2 | −/− | ‐ | MS | 453 |
| XG7 TP53 −/− #3 | −/− | ‐ | MS | >10,000 |
| XG7 TP53 R175H/R175H #1 | R175H/R175H | + (53 kDa) | MS | 661 |
| XG7 TP53 R175H/R175H #2 | R175H/R175H | + (53 kDa) | MS | 991 |
| XG7 TP53 R175H/R175H #3 | R175H/R175H | + (53 kDa) | MS | >10,000 |
Note: STP‐B IC50 value in 18 HMCLs and 13 TP53 isogenic clones from NCI‐H929 and XG7 was determined using a 72‐h CellTiter‐Glo assay. TP53 status (mutation and/or deletion), cytogenetic group, and p53 status are indicated.
Abbreviations: HMCL, human myeloma cell line; IC50, half‐maximal inhibitory concentration; ND, not determined; WB, western blot.